In Polytomella sp. mitochondria, biogenesis of the heterodimeric COX2 subunit of cytochrome c oxidase requires two different import pathways - ScienceDirect
Export JavaScript is disabled on your browser. Please enable JavaScript to use all the features on this page., May 2012, Pages 819-827Author links open overlay panelShow moreopen archiveAbstractIn the vast majority of eukaryotic organisms, the mitochondrial cox2 gene encodes subunit II of cytochrome c oxidase (COX2). However, in some lineages including legumes and chlorophycean algae, the cox2 gene migrated to the nucleus. Furthermore, in chlorophycean algae, this gene was split in two different units. Thereby the COX2 subunit is encoded by two independent nuclear genes, cox2a and cox2b, and mitochondria have to import the cytosol-synthesized COX2A and COX2B subunits and assemble them into the cytochrome c oxidase complex. In the chlorophycean algae Chlamydomonas reinhardtii and Polytomella sp., the COX2A precursor exhibits a long (130–140 residues), cleavable mitochondrial targeting sequence (MTS). In contrast, COX2B lacks an MTS, suggesting that mitochondria use different mechanisms to import each subunit. Here, we explored the in vitro import processes of both, the Polytomella sp. COX2A precursor and the COX2B protein. We used isolated, import-competent mitochondria from this colorless alga. Our results suggest that COX2B is imported directly into the intermembrane space, while COX2A seems to follow an energy-dependent import pathway, through which it finally integrates into the inner mitochondrial membrane. In addition, the MTS of the COX2A precursor is eliminated. This is the first time that the in vitro import of split COX2 subunits into mitochondria has been achieved.Graphical abstractHighlights? We developed a method to obtain import-competent mitochondria from Polytomella sp. ? The in vitro import of split COX2 subunits is described for the first time. ? COX2A follows an energy-dependent import pathway to the inner membrane. ? A lower hydrophobicity of the first transmembrane stretch of COX2A is critical. ? COX2B subunit is imported directly into the inter-membrane space.KeywordsPolytomella sp.Chlamydomonas reinhardtiiMitochondrial targeting signalCOX2Cytochrome c oxidase biogenesisRecommended articlesCiting articles (0)后使用我的收藏没有帐号？
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求艾斯剃ella cox全部作品，越多越好，谢谢
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